Table 3.

Nucleotide mutation and amino acid substitution in HPV-33 E6

	HPV-33 E6
No	1	2	3	4	5	6	7	8
Location	105	165	221	256	279	338	434	441
Mutation	A–C	A–G	G–C	A–C	A–C	A–G	G–T	T–C
Frequency (%)	19.44	7.87	3.24	7.41	19.44	7.41	15.28	11.57
Substitution	K35N	–	S74T	N86H	K93N	Q113R	R145I	–

Compared with the HPV-33 E6 reference sequence (M12732.1), the mutations are marked with the corresponding bases and amino acid, and those without changes are replaced with a dash (–). No. means the number of nucleotide mutations, Location means the sites of nucleotide mutations, Mutation means the style of nucleotide mutations, Frequency (%) means the percentage of nucleotide mutations, Substitution means the amino acid substitution that occurred by nucleotide mutations