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. 2022 Jan 20;22:83. doi: 10.1186/s12885-022-09183-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PFKFB3 knockdown inhibits RCC tumor growth in murine models. (a) Imaging of ACHN cell-based cancer formation in murines under different conditions. (b) Statistics for ACHN cell-based tumor volumes in nude murines (n = 6 per group). (c) Statistics for ACHN cell-based tumor weight in nude murines (n = 6 per group). (d) PFKFB3 mRNA levels were detected in tumors from different groups. (e) IHC imaging of PFKFB3 and Ki-67 in randomized ACHN cancer cells. (f) Quantification of IHC staining of Ki-67 in ACHN cell-based tumors. The results are presented as the mean ± SD. *, P < 0.05; **, P < 0.01