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. 2022 Jan 19;10(1):e004034. doi: 10.1136/jitc-2021-004034

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Major TIL subsets and the transcriptomic dynamics in MOC22 tumors with anti-PD1 treatment. A total of 5, 548 T cells were sequenced in MOC22 tumors treated with isotype control or anti-PD1. T cells from five mice in each treatment group were pooled and subjected to single cell sequencing. (A) UMAP of T cells in MOC22 tumors colored by individual clusters. (B) Violin plots showing expression of selected immune cell marker genes across clusters. The y axis represents the normalized gene expression levels. (C) UMAP of total T cells in MOC22 tumors colored by indicated major subsets. T cells from both conditions were pooled for clustering analysis. (D) Heatmap illustrating the relative gene expression levels of genes in major T cell subsets in MOC22 tumors. (E) UMAP of T cells in MOC22 tumors colored by treatment conditions. (F) Pseudobulk differential expression analysis was performed in total CD8 +T cells between anti-PD1 and isotype control treated MOC22 tumors. The results were presented by a color-coded volcano plot. The statistical significance (log10 FDR) was plotted against the log2 fold-change of gene expression levels. Each dot represents one gene, which is color coded by the most highly enriched genes. FDR, false discovery rates; MOC, murine oral carcinoma; TIL, tumor infiltrating lymphocytes.