FIG. 1.

Sensitivity of RT-PCR versus that of microplate virus isolation method for detection of BVDV. The gel shows amplicons from 10-fold serial dilutions in DEPC-treated water of RNA extracted from NADL strain BVDV. Lanes 1 to 8, 1:10¹ to 1:10⁸ dilutions, respectively; lane 9, kit control; lane M, molecular mass marker. The presence of 290-bp band indicated positivity for BVDV. Results were summarized in the row PCR across the bottom of the gel. The results of the microplate virus isolation method with the same serial 10-fold dilutions are listed in the row labeled VI.