Table 1.

Sequences of primers used for the detection of virulence genes in Escherichia coli isolates in the present study.

Target Gene	Pathotype	Primer Name	Primer Sequence (5′-3′)	Amplicon Size (bp)
Lambda	Internal Control	LF	CAGATCTCCAGCACGGAACTATTGAGTACGAACG	1000
		LR	GCATAAAATGCGGGGATTCACTGGCTGC
ipaH	EIEC	Ip-F	GATGCCGTGACAGCATGGTTCC	718
		Ip-R	CAGCCGGTCAGCCACCCTCTG
Vt1	EHEC	V1-F	GTCATTCGCCCTGCAATAGGTACTCC	599
		V1-R	AGTCTTGTCCATGATAGTCAGGCAGGAC
vt2	EHEC	V2-F	CGGACAGAGATATCGACCCCTCTTGAAC	481
		V2-R	CCTGACGAAATTCTCTCTGTATCTGCCTGAAG
bfpA	EPEC	Bf-F	CAGAATGCTATTTCAGAAGTAATGAGCGCAAC	400
		Bf-R	CAGTTGCCGCTTCAGCAGGAGTAATAG
aggR	EAEC	Ag-F	GGTCAAAAGGAATAATTGTAGCTGATGCTGACGAT	341
		Ag-R	GCTGCTTTGCTCATTCTTGATTGCATAAGGATCTGG
eaeA	EPEC	Ea-F	CCAGGCTTCGTCACAGTTGCAGGC	291
		Ea-R	CGCCACCAATACCTAAACGGGTATTATCACC
stp	ETEC	Sp-F	CCGTGAAACAACATGACGGGAGGTAACATGAAAAAGC	234
		Sp-R	GCACAGGCAGGATTACAACAAAGTTCACAGCAG
sth	ETEC	Sh-F	TTCACCTTTCGCTCAGGATGCTAAACCAG	171
		Sh-R	AGCACCCGGTACAAGCAGGATTACAACAC
lt	ETEC	Lt-F	GCAGGTTTCCCACCGGATCACCAAGC	127
		Lt-R	CAGATTCTGGGTCTCCTCATTACAAGTATCACCTG