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. 2021 Dec 28;11(1):67. doi: 10.3390/antiox11010067

Figure 5.

Figure 5

Effect of IFN I receptor knockout on AOE expression, antioxidant capacity, and markers of oxidative stress. Mice either sham PBS-inoculated or infected with RSV for 48 h were sacrificed to extract total lung RNA. Catalase, GST, and Prdx1 gene expression was quantified by q-RT-PCR (a). Data are expressed as means ± SD and normalized to WT PBS. Statistics were determined using Student’s t-test; ** p < 0.01 and * p < 0.05 relative to PBS-inoculated mice; # p < 0.05 relative to WT RSV-infected mice. BALF was collected on day 2 p.i. to measure (b) hydroxyl radical antioxidant capacity (gallic acid assay) and (c) advanced oxidation protein products (chloramine-T assay). Data are expressed as means ± SD. Statistics were determined using Student’s t-test; ** p < 0.01 and * p < 0.05 relative to PBS-inoculated mice; # p < 0.05 relative to WT RSV-infected mice. Data are representative of one of two independent experiments, with 4–6 animals/group.