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. 2022 Jan 15;11(1):164. doi: 10.3390/antiox11010164

Figure 7.

Figure 7

HPP and IP reduce proliferation and cytokine expression in ex vivo stimulated PBMC from patients with Inflammatory Bowel Disease. PBMC isolated from IBD patients were treated with (A,C) HPP or (B,D) IP (250 µM–1000 µM) for 6 h prior to stimulation with anti-CD3 for 12 h. After 18 h, culture media was replaced with fresh media and cells were incubated for a further 4 days with anti-CD3 stimulation. Supernatants were removed for analysis of cytokine concentration by ELISA. (A,B) Proliferation and cytokine production by CD3+CD8− cells was analysed by flow cytometry. Pooled data (N = 14) depicting the mean ± SEM of ki67 (as a measure of proliferation), IFNγ, and IL-17 in CD3+CD8− T cells. (C,D) Cell supernatants were assessed for concentrations of IL-10, IFNγ, and IL-17 by ELISA. Pooled data depicts mean (±SEM) cytokine concentrations for six IBD patients (means of three technical replicates per donor). Statistical significance was determined by repeated measures one-way ANOVA with Dunnett’s multiple comparisons post hoc test to compare means of treatment groups to the control group (*** p < 0.001, ** p < 0.01, * p < 0.05).