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. 2021 Dec 27;10(1):51. doi: 10.3390/biomedicines10010051

Figure 3.

Figure 3

ICOSL expression inhibits anchorage-independent tumor cell growth. (A) Kinetic growth assay of B16-ICOSL-low and B16-ICOSL-high cells in the presence of 10% FBS (n = 2). Cells were counted following trypan blue staining; (B) the soft agar colony formation assay was run to estimate anchorage-independent growth of B16-ICOSL-low and B16-ICOSL-high cells. Colonies were counted digitally using ImageJ software. Data are expressed as means ± SEM. The Mann–Whitney statistical test was used, ** p < 0.01.