Figure 6.

Deletion of VraR in MRSA ST30 strain showed reduced bacterial virulence. (A) Mice infected with wildtype ST30 or ST30ΔvraR. The skin lesion sizes were defined by darkened areas of necrosis and calculated by V = Πx (L × W)/2, V is the size of the lesion, L is the length of the lesion, and W is the width of the lesion. Hu depicts humanized mice. (B) Bacteria burden of mice after infection. The error bars represent the standard deviation of the mean values; significance was determined by one-way ANOVA, and coded as * p < 0.05. (C,D) Haematoxylin and eosin staining of the MRSA skin and soft-tissue infection mouse model. Mice were inoculated subcutaneously with either PBS or MRSA as described in Methods. (C) PBS control, (D) ST30 infected, and (E) ST30△vraR infected. Original magnifications of images are 100×.