Figure 1.

Viability counts for bacteria and fungi in the denture biofilm model. Denture biofilms formed on poly(methyl) methacrylate (PMMA) discs were treated for 7 days with six separate treatment modalities. These were non-treated controls (NT; blue bars), brushing with daily denture cleansing treatment (B + DC; pink bars), brushing only (+B; yellow bars), brushing with Colgate^® toothpaste (B + T1; light green bars) or brushing with Crest^® toothpaste (B + T2; dark green bars). The final treatment consisted of brushing with denture cleanser treatment on day 7 only (CFU counts for these are denoted by the dotted lines on the bar graphs). Results shown are representative of 2 individual experiments repeated with 3 separate replicates (n = 6), each for anaerobic bacteria (A), aerobic bacteria (B) and fungi (C). Heatmaps show the average log₁₀ fold reductions in CFU counts relative to NT. Significance values are shown as follows; * p < 0.05, ** p < 0.01 and *** p < 0.001, all calculated relative to the NT using the Kruskal–Wallis test with Dunn’s post-test.