Figure 4.

Effects of met-OLE on (A) intracellular ROS and (B) NO production and (C) overexpression of the iNOS, COX-2 and mPGES-1 proteins. Macrophages were pretreated with met-OLE (50, 25 or 12.5 μM) for 30 min and then, LPS-stimulated during 18 h. Subsequently, ROS and NO levels were measured using DCFDA and Griess assays in supernatants, respectively. The expression of iNOS, COX-2 and mPGES-1 proteins was then measured in whole cell lysates and analyzed by Western blotting. Results are presented as mean ± SEM of at least six independent experiments. + p < 0.05; ++ p < 0.01; +++ p < 0.001 vs. unstimulated control cells; ** p < 0.01; *** p < 0.001 vs. LPS-DMSO stimulated cells.