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. 2022 Jan 6;11(1):123. doi: 10.3390/antiox11010123

Figure 6.

Figure 6

meso-Dihydroguaiaretic acid (MDGA) reduces NET formation. (A) Neutrophils incubated with DMSO (0.1%) or MDGA (10 μM) and stimulated with PMA (10 nM) were stained with anti-elastase (red) and anti-myeloperoxidase (MPO, green) antibodies. Hoechst 33342 (blue) demonstrated DNA. Scale bars are 50 μm (n = 3). Scar bar: 50 µm. (B) Neutrophils were incubated with DMSO (0.1%) or MDGA (1, 3, and 10 μM) and then stimulated with PMA. The amount of free DNA was measured by SYTOX Green. All data are shown as the mean ± S.E.M.* p < 0.05 vs. activated control (n = 5).