Figure 7.

meso-Dihydroguaiaretic acid (MDGA) inhibits phosphorylation of ERK, JNK, and Akt, but not p38, SFKs in activated neutrophils. DMSO (0.1%) or MDGA (10 μM)-treated neutrophils were activated with or without fMLF. Phosphorylation of (A) ERK, (B) JNK, (C) Akt S473, (D) Akt T308, (E) p38, and (F) SFKs Y416 was assessed with immunoblotting using antibodies against the phosphorylated and native (total) forms of each protein. Blotted bands were analyzed with a densitometer, and the quantitative ratios of all samples were normalized to the corresponding total protein. All data are expressed as the mean ± S.E.M; (n = 5). * p < 0.05 vs. stimulated control.