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. 2022 Jan 15;11(1):143. doi: 10.3390/biology11010143

Figure 2.

Figure 2

Zika virus load in different astrocyte cell lines. Cells were infected with ZIKV strains MR766 and PRVABC59 at a multiplicity of infection (MOI) of 0.1. (A–C) Quantification of Zika virus RNA load in astrocyte cell subtypes using relative quantitative PCR. CCF-STTG1 astrocyte cells presented lowest ZIKV load at all timepoints compared with HASTR/ci35 and foetal-hTERT astrocyte cells. HASTR/ci35 cells showed higher viral RNA load (A–C) despite similar level viral entry compared with CCF-STTG1 (E). Focus-forming unit (FFU) assay was performed on supernatants collected at 24 h post-infection to assess the infection ability of the virus particles shed by the astrocyte cell lines. HASTR/ci35 astrocytes shed high-level active MR766 virus particles at 24 h compared with CCF-STTG1 and foetal-hTERT astrocytes (D). Data were normalised to the 36b housekeeping gene and expressed as a fold change relative to ZIKV RNA load at 6 h timepoints relative to mock-infected control (A–C) (data are shown as means ± SEM, n = 3). **** demonstrates significant differences between biological conditions by p < 0.0001.