Figure 4.

Proinflammatory (TNFα and IL-1β) and inflammatory (IP-10 and IL-8) genes showed a comparable expression pattern especially at earlier timepoints of ZIKV post-infection in CCF-STTG1 and HASTR/ci35 astrocyte cells. Cells were infected at an MOI of 0.1 and mRNA expressions were assessed at 24- and 48-h timepoints. Data were normalised to the 36b housekeeping gene and expressed as a fold change relative to mock-infected control (data are shown as means ± SEM, n = 3). Statistical analyses between two (C,D) and multiple (A,B) biological conditions were performed using one- and two-way ANOVA comparisons with Tukey and Sidak’s post hoc tests, respectively. *, **, *** and **** demonstrate significant differences between biological conditions by p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, subsequently.