Figure 5.

IFN-β and IFN-λ pre-stimulated ZIKV-susceptible HASTR/ci35 astrocyte cells partially control Zika virus strain PRVABC59 infection. Subconfluent ZIKV-susceptible HASTR/ci35 astrocyte cells were incubated with a low concentration of IFN-β (1000 U/mL) and IFN-λ (100 ng/mL) for 18 h followed by inoculation with PRVABC59 ZIKV strain at an MOI of 0.1. IFN-β showed effective control of the virus at an earlier timepoint but was unable to control persistently, whereas IFN-λ showed persistent but partial control of the virus. Cells were fixed at 24-, 48-, 72- and 148-h timepoints and then stained with 4G2 antibody to detect ZIKV envelope protein Env (stained green) and DAPI stain for cell nuclei (stained blue). Scale bars represent 200 µm.