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. 2022 Jan 15;11(1):143. doi: 10.3390/biology11010143

Figure 6.

Figure 6

The 0.3 µg/mL poly(dA:dT) and poly(I:C) pre-stimulated HASTR/ci35 astrocyte cells are able to control Zika virus strain PRVABC59 infection. Subconfluent ZIKV-susceptible HASTR/ci35 astrocyte cells were transfected with low concentrations (0.3 µg/mL) of poly(I:C) and poly(dA:dT) 18 h prior to infection with PRVABC59 ZIKV strain at an MOI of 0.1. Poly(I:C) showed complete control of the virus at this very low concentration whereas poly(dA:dT) was relatively less effective at this concentration. Cells were fixed at 24-, 48-, 72- and 144-h timepoints and then stained with 4G2 antibody to detect ZIKV envelope protein Env (stained green) and DAPI stain for cell nuclei (stained blue). Scale bars represent 200 µm.