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. 2021 Dec 28;11(1):62. doi: 10.3390/antiox11010062

Figure 4.

Figure 4

Outcome of treatment with 7,8-DHF in the neonatal period on PGC-1α expression in the hippocampal formation of Ts65Dn and euploid mice aged 15 days. Western blot analysis of PGC-1α expression in the hippocampus of P15 Ts65Dn and euploid mice treated either with vehicle (untreated mice) or 7,8-DHF (treated mice) during the postnatal days P3–P15. (A) Representative western blot images showing immunoreactivity for PGC-1α and the housekeeping gene α-Tubulin. (B) Levels of PGC-1α in the four experimental groups (untreated euploid and Ts65Dn mice and treated euploid and Ts65Dn mice). Values were normalized to α-Tubulin. Protein levels (mean ± SE) are expressed as a percentage of the values of untreated euploid mice. The scatterplots over each column represent the values of individual cases for each group. The following number of mice were analysed. Untreated euploid mice: n = 9; untreated Ts65Dn mice: n = 8; 7,8-DHF-treated euploid mice: n = 5; 7,8-DHF-treated Ts65Dn mice: n = 7. Two untreated euploid mice (yielding n = 7), one untreated Ts65Dn mouse (yielding n = 7), one 7,8-DHF-treated euploid mouse (yielding n = 4) and one 7,8-DHF-treated Ts65Dn mouse (yielding n = 6) were excluded from analysis based on the SPSS Box plot tool, as described in the Statistics section. *** p < 0.001 (Tukey’s test after two-way ANOVA). Abbreviations: 7,8-DHF, 7,8-dihydroxyflavone; α-Tub, α-Tubulin; Eu, euploid; Veh, vehicle.