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. 2022 Jan 20;42(1):e00327-21. doi: 10.1128/MCB.00327-21

FIG 6.

FIG 6

AMPKα controlled the expression of miR-206 by regulating HDAC4. (A and B) Western blotting of HDAC4 expression after FFA and AICAR cotreatment (n = 3). (C and D) Western blotting of HDAC4 expression after AICAR treatment (n = 3). (E and F) Western blotting of HDAC4 expression after compound C treatment (n = 3). (G and H) Western blotting of HDAC4 expression after AMPKα shRNA transfection (n = 3). (I) Relative mRNA expression of HDAC4 after HDAC4 siRNA transfection (n = 3). (J and K) Western blotting of HDAC4 expression after HDAC4 siRNA transfection (n = 3). (L) Relative miR-206 expression after HDAC4 siRNA transfection (n = 3). U6 was used as a control. (M) Western blotting of HDAC4, p-AMPKα, and AMPKα after cotreatment with AMPKα shRNA and HDAC4 siRNA (n = 3). (N) Relative miR-206 expression after cotreatment with AMPKα shRNA and HDAC4 siRNA (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars indicate SD.