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. 2022 Jan 20;42(1):e00327-21. doi: 10.1128/MCB.00327-21

FIG 7.

FIG 7

FFA damaged myogenesis through the AMPKα–HDAC4–miR-206 pathway. (A) Western blotting of AMPKα, p-AMPKα, and HDAC4 after cotreatment of AICAR with HDAC4 siRNA and the miR-206 inhibitor in FFA-treated C2C12 cells for 24 h (n = 3). (B) Relative miR-206 expression after cotreatment of AICAR with HDAC4 siRNA and the miR-206 inhibitor in FFA-treated C2C12 cells for 24 h (n = 3). (C) Western blotting of MyHC and myogenin after cotreatment of AICAR with HDAC4 siRNA and the miR-206 inhibitor in FFA-treated C2C12 cells for 24 h (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars indicate SD.