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Primer sequences for RT-qPCR
| Primer or gene name | Primer directiona | Primer sequence (5′–3′) |
|---|---|---|
| miR-206-specific primer | TCGGCAGGTGGAATGTAAGG | |
| Myogenin | F | TGGAGCTGTATGAGACATCCC |
| R | TGGACAATGCTCAGGGGTCCC | |
| MyOD1 | F | GCCCGCGCTCCAACTGCTCTGAT |
| R | TCTTTTGGGCGTGAAGAACCAG | |
| GAPDH | F | ATCACTGCCACCCAGAAGACT |
| R | CATGCCAGTGAGCTTCCCGTT | |
| HDAC4 | F | CTGCAAGTGGCCCCTACAG |
| R | CTGCTCATGTTGACGCTGGA | |
| Cyclin D1 | F | CACCGGCCTCTGGCTAAAC |
| R | CGCAGGCTTGACTCCAGAAG | |
F, forward; R, reverse.
