Figure 1.

CLA pretreatment ameliorated the accumulation of reactive oxygen species (ROS) caused by LPS challenge in BMECs. (a) BMECs distribution diagram marked with green fluorescence probe DCFH-DA, which accurately represents the ROS concentration precisely. (b) The fluorescence intensity histogram, and the median of the fluorescence intensity was statistically analyzed. The data are presented as the means ± SEM. Experiments were repeated three times independently with triplicates in each treatment. * p ≤ 0.05, significantly different from CON; # p ≤ 0.05, significantly different from LPS. (c) ROS was marked with green fluorescence probe DCFH-DA and visualized by laser confocal microscopy. The T-PMT model was used to observe cell morphology and positioning.