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. 2021 Dec 27;11(1):55. doi: 10.3390/antiox11010055

Figure 2.

Figure 2

CLA pretreatment alleviated oxidative stress induced by LPS challenge in BMECs. (a–d) mRNA abundance of antioxidative genes (e–h) the activity of anti-oxidative enzymes by commercial kits (i) immunoblotting of HMOX1 and Nrf2 by Western blotting (j) Nrf2 visualized by FITC fluorescence (green), and nuclei visualized by DAPI (blue) through immunofluorescence analysis were measured to study the effect of CLA pretreatment and LPS challenge on the redox state of BMECs. The data are presented as the means ± SEM. Experiments were repeated three times independently with triplicates in each treatment. * p ≤ 0.05, significantly different from CON; # p ≤ 0.05, significantly different from LPS.