Figure 5.

CLA pretreatment relieved the autophagy level stimulated by LPS challenge in BMECs. (a) Immunoblotting of autophagy related protein, LC3, p62, beclin 1 and ATG5 was measured by Western blotting. GAPDH was the reference protein to normalize the result of target proteins. The data are presented as means ± SEM. Experiments were repeated three times independently with triplicates in each treatment. * p ≤ 0.05, significantly different from CON; # p ≤ 0.05, significantly different from LPS. (b) p62 expression stained with FITC fluorescence (green) was visualized by immunofluorescence, and nuclei were stained with DAPI (blue).