Figure 6.

LPS and H₂O₂ treatment depressed mRNA abundance of milk fat synthesis-related genes in BMECs. (a) The mRNA abundance of milk fat synthesis-related transcription factor (SREBP1 and PPARG) and milk fat synthase (ACACA, FASN and SCD) in BMECs was measured under treatment with 8μg/mL LPS for 0, 2, 4, 6, 8, 12 and 24 h. (b) Oxidative stress related transcription factors Nrf2, milk fat synthesis-related transcription factors (SREBP1 and PPARG) and milk fat synthases (ACACA and FASN) were measured after BMECs were treated with 50 μM H₂O₂ for 1, 3 and 6 h and 1, 10, 50 and 100 μM H₂O₂ for 6 h. GAPDH was the housekeeping gene and the data are presented as means ± standard error of mean (SEM). Experiments were repeated three times independently with triplicates in each treatment. * p ≤ 0.05, significantly different from CON (0 h).