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. 2021 Dec 23;10(1):17. doi: 10.3390/biomedicines10010017

Table 1.

Main characteristic of BBB4-G4K NPs [16].

Analysis BBB4-G4K NPs
FTIR
[cm−1]		 Bands Attribution
3500–3000 (NH3+ dendrimer, OH stretching BBB4)
2985, 2880 (alkyl groups of dendrimer and BBB4)
1736 (C=O stretching esters of dendrimer)
1220, 1051 (C-O stretching esters of dendrimer)
697 (C-Br stretching of BBB4)		 graphic file with name biomedicines-10-00017-i001.jpg
1H NMR
(400 MHz, CD3OD)
[ppm]		 Signals attribution
1 = 423 H (CH3 of G4K + CH2CH2CH2 of lys)
2 = 96 H (CH2NH3+ of lys)
3 = 120H (CH2 of BBB4 + CHNH3+ of lys + CH2 of BBB4)
4 = 186 H (CH2O of G4K)
5 = 144 H (CH= of phenyl rings)
6 = 36 H (CH= of phenyl rings)		 graphic file with name biomedicines-10-00017-i002.jpg
HPLC DL (%)
EE (%)		 28.8 ± 1.2
39.0 ± 1.6
1H NMR MW 21,175.8
DL% by HPLC 21,072.6 ± 240.2
Scanning Electron Microscopy (SEM) Morphology
Average Size		 graphic file with name biomedicines-10-00017-i003.jpg
DLS 1 Analysis Z-Ave 2,5 (nm)
PDI 3,5 		graphic file with name biomedicines-10-00017-i004.jpg
ζ-p 4,5 (mV) graphic file with name biomedicines-10-00017-i005.jpg
Solubilization Essay § Water-Solubility (mg/mL) graphic file with name biomedicines-10-00017-i006.jpg
Dialysis Method (HPLC) Cumulative Release (%, 24 h) graphic file with name biomedicines-10-00017-i007.jpg
Mathematical Model graphic file with name biomedicines-10-00017-i008.jpg
Mechanism Drug release rate is concentration-dependent
G4K Cytotoxicity
(HeLa Cells)		 Cell Viability (%)
(0–100 µM)		 graphic file with name biomedicines-10-00017-i009.jpg
Potentiometric Titration # Protonation Profile graphic file with name biomedicines-10-00017-i010.jpg

1 dynamic light scattering; 2 hydrodynamic diameters of particles; 3 polydispersity indices; 4 measure of the electrical charge of particles suspended in the liquid of acquisition (water); 5 correspondent values for G4K = 333.4 ± 24.6, 0.286 ± 0.040, +66.1 ± 4.7; § the image also shows the water solubility of untreated BBB4, nanoengineered BBB4 released in water, and BBB4 contained in NPs; Paclitaxel = positive control; # the image also shows the titration curve and the first derivative of G4K.