Table 3.
MICs of BBB4-G4K NPs, expressed as µg/mL and micromolarity (µM), against the Staphylococcus species tested in the study; the relative maximum concentrations of BBB4 that should be released by BBB4-G4 NPs according to the observed MICs; and the relative DL% and the release profile [16]. The MICs of pristine BBB4 were also reported. The last two columns report the values of selectivity indices (SI) of BBB4 and BBB4-G4K NPs.
| BBB4 MW 343.2 |
BBB4-G4K MW 21,175.8 |
Max BBB4 Releasable 1 | Oxacillin | SI2 BBB4 |
SI2 BBB4-G4K |
|
|---|---|---|---|---|---|---|
| MIC Values 3 | µg/mL, µM | µg/mL, µM | µg/mL, µM | µg/mL, µM | ||
| S. aureus 17 * | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. aureus 18 * | 16, 46.6 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 1.2 | 2.7 |
| S. aureus 191 * | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. aureus 195 * | 32, 93.2 | 32, 1.5 | 8.8, 25.7 | 256, 637.8 | 0.6 | 5.5 |
| S. aureus 292 * | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. aureus 293 * | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. aureus 414 | 16, 46.6 | 32, 1.5 | 8.8, 25.7 | <2.0, <5.0 | 1.2 | 5.5 |
| S. aureus 187 * | 16, 46.6 | 32, 1.5 | 8.8, 25.7 | 256, 637.8 | 1.2 | 5.5 |
| S. aureus 188 * | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. aureus 26 * | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. epidermidis 22 § | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. epidermidis 180 § | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. epidermidis 181 †,§ | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. epidermidis 197 § | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. epidermidis 198 †,§ | 32,93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6 | 2.7 |
| S. epidermidis 222 § | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | 512, 1275.5 | 0.6. | 2.7 |
| S. haemoliticus 193 †† | >128, >373.0 | 128, 6.0 | 35.2, 102.6 | 512, 1275.5 | <0.15 | 1.4 |
| S. hominis 125 †† | >128, >373.0 | 128, 6.0 | 35.2, 102.6 | 512, 1275.5 | <0.15 | 1.4 |
| S. lugdunensis 129 | 128, 373.0 | 64, 3.0 | 17.6, 51.3 | <2.0, <5.0 | 0.15 | 2.7 |
| S. saprophyticus 41 | 32, 93.2 | 64, 3.0 | 17.6, 51.3 | <2.0, <5.0 | 0.6 | 2.7 |
| S. symulans 163 †† | >128, >373.0 | 128, 6.0 | 35.2, 102.6 | 512, 1275.5 | <0.15 | 1.4 |
| S. warneri 74 †† | 64, 186.5 | 64, 3.0 | 17.6, 51.3 | 256, 637.8 | 0.3 | 2.7 |
1 Derived from the MICs observed for BBB4-G4K NPs after 24 h and according to their DL% and the BBB4 released from BBB4-G4K NPs after 24 h [16]; 2 SI = selectivity index (LD50/MIC) where LD50 was calculated using data from cytotoxicity experiments on human keratinocytes after 24 h exposure; 3 experiments were performed in triplicate, the concordance degree was 3/3, and ±SD was zero; * indicates MRSA; § indicates MRSE; † indicates MRSE also resistant to linezolid; †† indicates resistance to oxacillin.