Figure 5.

Effects of GYY4137 treatment on the emotional disorders accompanying PTX-induced neuropathic pain. Effects of the repetitive administration of 35 mg/kg of GYY4137 or vehicle from days 18 to 21 after PTX or vehicle (VEHI) injection on the anxiety- (EPM and OF tests) and depressive-like behaviors (TST and FST) associated with CIPN. In the EPM test, the number of times that animals entered into the open arms (A), the percentage of total time spent in open arms (B), and the number of entrances into the closed arms (C) are represented. In the OF test, graphs show the number of entries into the central area (E), the percentage of time spent within the central area (F), and the total number of squares crossed (G). Figures (D,H) display the time spent in immobility (s) in the TST (D) and the FST (H). In all panels, * indicates significant differences vs. the vehicle–vehicle-treated mice; &, vs. the vehicle–GYY4137-treated animals; and $, vs. the PTX–GYY4137-treated animals (p < 0.05, one-way ANOVA followed by the SNK post hoc test). Data are expressed as mean values ± SEM; n = 8 animals per experimental group.