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. 2022 Jan 7;10(1):132. doi: 10.3390/biomedicines10010132

Figure 6.

Figure 6

Overexpression of huChem-163 in human LX-2 cells: (A) Immunoblot of chemerin, alpha-smooth muscle actin (alpha-SMA), and galectin-3 in LX-2 cells overexpressing huChem-163; (B) Chemerin in the supernatant of these cells (n = 4); (C) Activation of human chemokine-like receptor 1 (CMKLR1) and human G protein-coupled receptor 1 (GPR1) by the media of the recombinant cells relative to total cell media chemerin. Control-transfected cell supernatant was not active, and overexpression of huChem-163 did not significantly increase CMKLR1 or GPR1 activity (n = 4); (D) Lactate dehydrogenase (LDH) activity (n = 4; arbitrary units: a.u.); (E) Number of live cells (n = 3); (F) Number of dead cells (n = 3). Student’s t-test, ** p < 0.01.