Figure 9.

Requirement of protein synthesis in YRL1091-induced cytoplasmic vacuolization and cell death in MDA-MB-231 and MCF-7 cells. (A–C) Cells were treated with 30 µM YRL1091 in the presence or absence of CHX (2 µM, pretreatment for 2h) for 24 h. (A) Levels of LC3B were measured by western blotting. Each data point was calculated as the percentage of protein level in vehicle-treated control cells. Representative bands from at least three independent experiments were shown. (B) Phase-contrast images were captured. Scale bar: 20 µm. (C) Cell viability was determined by SRB assay at 24 and 48 h. The cell viability of each treated group was expressed as the percentage of that in control group. Data are presented as the mean ± SEM from at least three independent experiments.* p < 0.05 vs. vehicle-treated control cells; # p < 0.05 vs. YRL1091-treated cells.