Figure 7.

Apoptosis, necrosis, and senescence in canine cells. Boxplots display (A) apoptosis levels of canine mesenchymal stromal cells (MSC), measured by a luminescence-based Annexin V assay in passage 3 (P3) and 5 (P5) at day 5; (B) necrosis levels of canine MSC, measured using a cell-impermeant and pro-fluorescent DNA dye in passage 3 (P3) and 5 (P5) at day 5; (C) senescence levels of canine MSC in passage 3 (P3) and 5 (P5) at day 5, measured based on SA-β-galactosidase activity. Friedman tests for group comparisons and subsequent post hoc tests were performed; the asterisks indicate the significant differences between the corresponding groups (* corresponds to p < 0.05). Data were obtained using MSC from the same n = 5 donor dogs in each group.