Table 2.
Real-time PCR primer sequences.
| Genes | Forward (5′→3′) | Reverse (5′→3′) | Temperature (°C) | Accession Number |
|---|---|---|---|---|
| CuZnSOD | CGCACTTCAACCCTTACA | ACTTTCCTCATTGCCTCC | 61.5 | GU901214 |
| MnSOD | ACGACCCAAGTCTCCCTA | ACCCTGTGGTTCTCCTCC | 60.4 | GU218534 |
| CAT | GAAGTTCTACACCGATGAGG | CCAGAAATCCCAAACCAT | 58.7 | FJ560431 |
| GPx1a | GGGCTGGTTATTCTGGGC | AGGCGATGTCATTCCTGTTC | 61.5 | EU828796 |
| GPx1b | TTTTGTCCTTGAAGTATGTCCGTC | GGGTCGTTCATAAAGGGCATT | 60.3 | KT757315 |
| GPx4a | TACGCTGAGAGAGGTTTACACAT | CTTTTCCATTGGGTTGTTCC | 60.4 | KU255598 |
| GPx4b | CTGGAGAAATACAGGGGTTACG | CTCCTGCTTTCCGAACTGGT | 60.3 | KU255599 |
| GSTr | TCTCAAGGAACCCGTCTG | CCAAGTATCCGTCCCACA | 58.4 | EU107283 |
| GSTp1 | ACAGTTGCCCAAGTTCCAG | CCTCACAGTCGTTTTTTCCA | 59.3 | KM112099 |
| GSTp2 | TGCCTTGAAGATTATGCTGG | GCTGGCTTTTATTTCACCCT | 59.3 | KP125490 |
| GR | GTGTCCAACTTCTCCTGTG | ACTCTGGGGTCCAAAACG | 59.4 | JX854448 |
| Nrf2 | CTGGACGAGGAGACTGGA | ATCTGTGGTAGGTGGAAC | 62.5 | KF733814 |
| keap1a | TTCCACGCCCTCCTCAA | TGTACCCTCCCGCTATG | 63.0 | KF811013 |
| keap1b | TCTGCTGTATGCGGTGGGC | CTCCTCCATTCATCTTTCTCG | 57.9 | KJ729125 |
| TOR | TCCCACTTTCCACCAACT | ACACCTCCACCTTCTCCA | 61.4 | JX854449 |
| S6K1 | TGGAGGAGGTAATGGACG | ACATAAAGCAGCCTGACG | 54.0 | EF373673 |
| β-actin | GGCTGTGCTGTCCCTGTA | GGGCATAACCCTCGTAGAT | 61.4 | M25013 |
CuZnSOD = copper/zinc superoxide dismutase; MnSOD = manganese superoxide dismutase; CAT = catalase; GPx1a = glutathione peroxidase 1a; GPx1b = glutathione peroxidase 1b; GPx4a = glutathione peroxidase 4a; GPx4b = glutathione peroxidase 4b; GSTr = glutathione-S-transferase r; GSTp1 = glutathione-S-transferase p1; GSTp2 = glutathione-S-transferase p2; GR = glutathione reductase; Nrf2 = nuclear factor erythroid 2-related factor 2; Keap1a = Kelch-like ECH-associated protein 1a; Keap1b = Kelch-like ECH-associated protein 1b; TOR = target of rapamycin; S6K1 = ribosomal protein s6 kinase polypeptide 1.