Figure 6.

EIS measurement for monitoring of hiPSC-derived BCECs culture over 168 h in dynamic and static conditions (biological replicates N = 7 (0 h–48 h) and N = 3 (72 h–168 h)). (a) Equivalent circuit design based on the bioreactor culture system: CPE constant phase element of electrode, Cc: capacitance of cell membrane, R_T: Resistance of tight junction barrier (TEER_simulation), R_m: resistance of culture medium; (b) results of fitted TEER_simulation; and (c) cell membrane capacitance obtained by fitting and simulation of impedance data. Graphs show a comparison between dynamic and static culture over the period of culture.