Aggressive behaviors were compared across four time points using least square means regression analysis. Aggressive behaviors were recorded using duration (seconds (s)) then log10 + 1 transformed. The four time points are: immediately after mix, and 3, 6, 9 weeks (wk) after mix. Observations at 6 wk were made after pigs were moved to a new pen with their group. Aggression is presented as total duration (s) of aggression and total duration (s) of initiated aggression. Errors bars represent the 95% confidence interval of the least square means. a–d: Bars with different letters denote time periods that showed significantly different durations of that behavior (p < 0.05, Tukey, HSD adjusted).