Table 3.
Sequences and relative positions of primers for target genes used in real-time qPCR.
| Gene | Sequence | Amplicon bp | Accession No. * | References |
|---|---|---|---|---|
| GAPDH | F: 5′- GCTGGCATTGCACTGAATGAC -3′ | 113 | NM_204305.1 | [4] |
| R: 5′- CACTCCTTGGATGCCATGT -3′ | ||||
| ACTB | F: 5′- AGCGAACGCCCCCAAAGTTCT -3′ | 139 | NM_205518.1 | [4] |
| F: 5′- AGCTGGGCTGTTGCCTTCACA -3′ | ||||
| CAT | R: 5′- TGGCGGTAGGAGTCTGGTCT -3′ | 112 | NM_001031215.1 | [4] |
| R: 5′- GTCCCGTCCGTCAGCCATTT -3′ | ||||
| GPX1 | F: 5′- AACCAATTCGGGCACCAG -3′ | 122 | NM_001277853.2 | [4] |
| R: 5′- CCGTTCACCTCGCACTTCTC -3′ | ||||
| GPX2 | F: 5′- GAGCCCAACTTCACCCTGTT -3′ | 75 | NM_001277854.2 | [4] |
| R: 5′- CTTCAGGTAGGCGAAGACGG -3′ | ||||
| SOD1 (CuZn) | F: 5′- CACTGCATCATTGGCCGTACCA -3′ | 224 | NM_205064.1 | [4] |
| R: 5′- GCTTGCACACGGAAGAGCAAGT -3′ | ||||
| GSTA2 | F: 5′- GCCTGACTTCAGTCCTTGGT -3′ | 138 | XM_015284825.3 | [4] |
| R: 5′- CCACCGAATTGACTCCATCT -3′ | ||||
| NOS2 | F: 5′- AAAGAAAGGGATCAAAGGTGGT -3′ | 296 | NM_204961.1 | [4] |
| R: 5′- CAAGCATCCTCTTCAAAGTCTG -3′ | ||||
| NOX1 | F: 5′- TCATCACTCTGGCGCTCATC -3′ | 171 | XM_040698828.1 | [4] |
| R: 5′- CCTTCATGCTCTCCTCCGTC -3′ | ||||
| NOX2 | F: 5′- TGGTGCGGTTTTGGAGATCA -3′ | 145 | XM_040698636.1 | [4] |
| R: 5′- GACACTGCTGGGCATTTGAC -3′ | ||||
| NOX3 | F: 5′- TTGGAATGGGAGAAGGCCAC-3′ | 92 | XM_040667279.1 | [4] |
| R: 5′-AGCACCACAGGACTCACAAC-3′ | ||||
| TLR4 | R: 5′- ACCCGAACTGCAGTTTCTGGAT -3′ | 120 | NM_001030693.1 | [27] |
| R: 5′- AGGTGCTGGAGTGAATTGGC -3′ | ||||
| MAPK | F: 5′- GAACGTGCGCTTCATCTACG -3′ | 137 | XM_040649449.1 | [27] |
| R: 5′- CCACGGGCTTAAACGCTTTC -3′ | ||||
| NFKB | F: 5′- GAAGGAATCGTACCGGGAACA -3′ | 131 | NM_205134 131 | [27] |
| R: 5′- CTCAGAGGGCCTTGTGACAGTAA -3′ | ||||
| TNF | F: 5′- CCCCTACCCTGTCCCACAA -3′ | 67 | NM204267 | [27] |
| R: 5′- TGAGTACTGCGGAGGGTTCAT -3′ | ||||
| INFA | F: 5′- ACTTCAGCTGCCTCCACACCTT -3′ | 92 | AM049251.1 | [27] |
| R: 5′- CAGGAACCAGGCACGAGCTT -3′ | ||||
| INFG | F: 5′- AACAACCTTCCTGATGGCGTGA -3′ | 89 | NM_205149.1 | [27] |
| R: 5′- GCTTTGCGCTGGATTCTCAAGT -3′ | ||||
| IL1B | F: 5′- TGCTTCGTGCTGGAGTCACCC -3′ | 98 | XM_015297469.1 | [27] |
| R: 5′- GGCCGGTACAGCGCAATGTT -3′ | ||||
| IL2 | F: 5′- CGTAAGTGGATGGTTTTCCTCT -3′ | 161 | NM204153 | [27] |
| R: 5′- GGCTAAAGCTCACCTGGGTC -3′ | ||||
| IL6 | F: 5′- AGCGAAAAGCAGAACGTCGAGTC -3′ | 107 | XM_015281283.2 | [27] |
| R: 5′- GCCGAGTCTGGGATGACCACTTC -3′ | ||||
| IL8 | F: 5′- CTGGCCCTCCTCCTGGTT-3′ | 105 | HM179639 | [27] |
| R: 5′- GCAGCTCATTCCCCATCTTTAC -3′ | ||||
| IL18 | F: 5′- GTTGTTCGATTTAGGGAAGGAG -3′ | 146 | NM204608.1 | [27] |
| R: 5′- TCAAAGGCCAAGAACATTCC -3′ |
* Ref Seq: NCBI Reference Sequence database.