Figure 4.

Effects of the HP0044- and HP1275-disrupted mutations on H. pylori growth, sensitivity to the detergent SDS and resistance to the antibiotic novobiocin. (a) The growth curves of wild-type strain 26695, the HP0044 and HP1275 knockout mutants and their corresponding knockout complementary mutants. The data were recorded by a spectrophotometer at ${\mathrm{OD}}_{600}$ at different time points. The data are presented as the average ${\mathrm{OD}}_{600}$ values derived from triplicate trials with statistical analysis (unpaired, two-tailed Student’s t-test; * p < 0.05). (b) SDS sensitivity and (c) novobiocin resistance of the tested H. pylori strains. The effects of the HP0044 and HP1275 mutations on SDS sensitivity and novobiocin resistance of H. pylori were evaluated by the survival rate, which was measured by the ratio of the ${\mathrm{OD}}_{600}$ of bacteria incubated with/without various concentrations of SDS and novobiocin. The data presented were derived from triplicate trials with statistical analysis (unpaired, two-tailed Student’s t-test; * p < 0.05 and *** p < 0.001). WT, wild-type strain; KO, knockout mutant; and Com, knockout complementary mutant.