Table 2.
Sequences and restriction sites of the primers used in this study.
| Primer | Restriction Enzyme Site | Sequence (5′→3′) 5 |
|---|---|---|
| KO0044F1 1 | - | AATCGCTTTAATCACCGGGG |
| KO0044R1 1 | BamHI | TACAGGATCCAAAGTTTCGC |
| KO0044F2 1 | BamHI | CTTTGGATCCTGTAACCCGT |
| KO0044R2 1 | - | ATGCCATAGGCACCAGTGAT |
| Com0044F1 2 | NcoI | CCATGGCTTGATTGGAAGCACTAGCCACG |
| Com0044R1 2 | - | ATTTTTTCTTTCATATCGTAACTCCTTAAGTGTT |
| Com0044F2 2 | - | TAAGGAGTTACGATATGAAAGAAAAAATCGCTTT |
| Com0044R2 2 | KpnI | AAGGTACCTCAGTGGTGATGGTGATGATGTTCATAAAAATTCCT |
| KO1275F1 3 | - | AAACGCATGGCAAAATTTATGCG |
| KO1275R1 3 | BamHI | CCCAGGATCCTCAGGATCCG |
| KO1275F2 3 | BamHI | CTGAGGATCCTGGGAATTTC |
| KO1275R2 3 | - | GGGATATGAAATGAGATTATCCC |
| Com1275F1 4 | NcoI | CCATGGCTTGATTGGAAGCACTAGCCACG |
| Com1275R1 4 | - | ATGCTAATGTCCATATCGTAACTCCTTAAGTGTT |
| Com1275F2 4 | - | TAAGGAGTTACGATATGGACATTAGCATTTTTAG |
| Com1275R2 4 | KpnI | AAGGTACCTTAGTGGTGATGGTGATGATGAAGTTTTTCTAATAA |
1 Primers used in the construction of the HP0044 knockout mutant. 2 Primers used in the construction of the HP0044 knockout complementary mutant. 3 Primers used in the construction of the HP1275 knockout mutant. 4 Primers used in the construction of the HP1275 knockout complementary mutant. 5 The sequences of restriction enzyme sites are underlined.