Skip to main content
. 2022 Jan 8;10(1):136. doi: 10.3390/biomedicines10010136

Figure 5.

Figure 5

Deregulated microRNAs (miRNAs) after lateral fluid percussion injury. (A) Unsupervised hierarchical clustering partly separated sham-operated controls from post-TBI rats according to differentially expressed (D,E) miRNAs in the perilesional cortex. A total of 12 upregulated and 7 downregulated miRNAs were found (FDR <0.05). (B1–B4) Droplet digital PCR (ddPCR) analysis validated miR-375 (B1, p < 0.01) and miR-211 (B2, p < 0.05) upregulation. In the ipsilateral thalamus, only 2 miRNAs were upregulated in small non-coding RNAseq analysis (sncRNAseq, FDR <0.05). Both of these were validated with ddPCR (B3, miR-146a, p < 0.05; B4, miR-155, p < 0.01). (C) All other sncRNAseq suggested no change in the top 10 DE miRNAs in ddPCR analysis (p > 0.05 for all). Most of them (7/8), however, were regulated in the same direction detected in the sncRNAseq analysis. (D) Functional pathway analysis of predicted targets of miR-375 and miR-211. (E) Functional pathway analysis of predicted targets of miR-146a and miR-155. (F1,F2) Quantitative RT-PCR analysis revealed no change in the predicted targets selected for validation (ELAV Like RNA Binding Protein 2 (Elavl2) and Synaptotagmin 1 (Syt1), p > 0.05). Statistical significance: *, p < 0.05; **, p < 0.01.