Figure 2.

Phospho-p53 and p21 protein expression in GCD@DOX-, GCD- and DOX-treated cells. HEp-2 cells were treated or untreated with 25 μg/mL of GCD@DOX for the indicated time (24 h, 48 h and 72 h). DOX and GCD were used as controls. (A) An equal amount of proteins was separated by polyacrylamide gel electrophoresis and probed with a specific antibody to phospho-p53 and p21. GAPDH was used as housekeeping gene. (B) The quantitative densitometric analysis for phospho-p53 and p21 band intensities was determined using ImageJ software and expressed as fold change over the appropriate housekeeping gene.