Figure 3.

Removal of GLI3 or GLI2/GLI3 does not alter olfactory interneuron production in the dorsal V-SVZ: Ad-GFAPp-Cre virus was injected in dorsal V-SVZ in CAG, Gli3^FLX/FLX; CAG, or Gli2^FLX/FLX; Gli3^FLX/FLX; CAG animals, and brain tissue was analyzed 30 days after injection. Approximate locations of the imaged areas are indicated by red boxes. GFP/GFAP double-labeled cells were found at the injection site (A,D,K,N), and GFP-labeled progeny were scored in the olfactory bulb for all sites and genotypes (B,E,L,O; GFP alone shown in C,F,M,P). Scale bars—50 microns; red dashed lines indicate the core of the olfactory bulb. Ablation of GLI3 in the dorsal V-SVZ did not alter the fraction of GFP-positive cells that also incorporated BrdU (G–I). No difference in the location of interneurons in the granular layer was seen for any genotype (J,R). Ventral injection in either Gli3^FLX/FLX; CAG animals or Gli2^FLX/FLX; Gli3^FLX/FLX; CAG animals resulted in a substantial reduction in the number of interneurons per tissue slice in the olfactory bulb; this was not seen after dorsal injections (Q).