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. 2022 Jan 7;14(2):282. doi: 10.3390/cancers14020282

Figure 3.

Figure 3

Inducible HPV16E6E7 knockdown refractory cells have lower copies of HPV16E6E7 shRNA. UMSCC47-shRNA4 cells were unstimulated or stimulated with doxycycline for 20 continuous days to generate a paired parental and refractory cell line. (A) Colony formation assay. Parental and refractory cells were plated and treated with control or doxycycline for 10 days. Representative cell culture plates are shown for each experimental condition. (B) Whole exome sequencing. Exonic SNVs were unchanged between UMSCC47-shRNA parental and refractory cells.