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Inducible HPV16E6E7 knockdown alters the transcriptomic signature of HPV16+ SCC cells. (A) Volcano plot. Total RNA was extracted from UMSCC47-shRNA4 cells at day 0 and at days 2, 4, and 6 after doxycycline induction using TRIZol and processed for RNA-Seq. Transcriptomic and KEGG pathway analyses were performed with the GAGE package in R. (B) Heat map of representative genes in the p53 and PPAR pathways.
