Skip to main content
. 2022 Jan 6;11(2):182. doi: 10.3390/cells11020182

Figure 2.

Figure 2

Chronic alcohol consumption and Nlrp6 deficiency modulate intestinal epithelium in mice. (a,b) Representative images of histological colon sections stained with periodic acid-Schiff (PAS) (a) or stained for MUC2 (green) and nuclei (DAPI, blue) (b) from ethanol-fed and control-fed WT and Nlrp6−/− deficient mice (scale bars, 100 µm, with area indicated by rectangle frame shown enlarged below “Detail”). (c–f) Relative mRNA expression of Muc2 in ileum (c), Tjp1 in ileum and colon (d), Cldn2 in ileum and colon (e), and Reg3g in ileum and colon (f) was determined by RT-qPCR analysis of tissues from ethanol-fed and control-fed WT and Nlrp6−/− mice and normalized to B2m. Data represent mean ± SEM of at least five mice per group; ** p < 0.01, * p < 0.05.