Figure 8.

Variation in velocity descriptors (VCL and VSL) in hyperactivated and non-hyperactivated spermatozoa from three mouse species (M. musculus, M. spretus, M. spicilegus) under capacitating conditions (mT-BH medium under 5% CO₂/air). Data are means ± SE (n = 7 in M. musculus, n = 8 in M. spretus, and n = 6 in M. spicilegus). Light blue triangles: non-hyperactivated sperm in capacitating conditions. Dark blue triangles: hyperactivated sperm in capacitating conditions. (A–C) Curvilinear velocity (VCL) (µm s⁻¹), (D–F) Straight-line velocity (VSL) (µm s⁻¹). (A,D) M. musculus. (B,E) M. spretus. (C,F) M. spicilegus. Asterisks indicate significant differences (p < 0.05) between non-hyperactivated and hyperactivated sperm for the same time in a Fisher post-hoc test. Different letters between times of incubation for the same status (hyperactivated or non-hyperactivated) indicate statistically significant differences (p < 0.05) in a Fisher post-hoc test. If two time-points of an incubation share any letter, the differences between them are not significant. No letters were used when a time-point of an incubation did not show any significant effect in the dependent variable.