Figure 2.

Effects of Amb544925 on [³H]choline uptake, cell viability and caspase 3/7 activity in HSC-3 cells. (A) Effect of Amb544925 on 10 µM [³H]choline uptake (n = 3). Cells were preincubated with each concentration of Amb544925 for 20 min, and then 10 µM [³H]choline uptake was added and uptake was measured for 20 min. The uptake of [³H]choline in the Amb544925 treatment was expressed as a percentage of that in the vehicle control. The IC₅₀ value calculated from the four-parameter logistic curve was 6.1 μM. (B) Effect of Amb544925 on cell viability. Cells were incubated with each concentration of Amb544925 for 24, 48, and 72 h, and then cell viability was measured (n = 3). Results are presented as a percentage of the vehicle control. Effect of 30 µM Amb544925 on cell viability (C) and caspase-3/7 activity (D). Cells were incubated with 30 µM Amb544925 for 8, 10, and 12 h, and then cell viability and caspase-3/7 activity were measured simultaneously (n = 4). The results were presented as a percentage of the vehicle control. * p < 0.05 and *** p < 0.005 compared to the vehicle control.