Figure 6.
Experimental design for MS experiments. (A) Northern blot for the detection of PSTVdNB in N. benthamiana plants. Total RNA staining (methylene blue) was used as loading control. (B) Three different strategies were followed in this study. In strategy 1, total lysate from both infected and non-infected plants was used for further MS analysis. In strategy 2, total lysate was filtered through specialized column to keep only small peptides, and then proceed with MS analysis. In strategy 3, a 15% polyacrylamide gel was used to separate proteins and only proteins smaller than 30 kDa were kept for further MS analysis.