Table 1.
List of primers for RT-qPCR analysis. The primer sequences for collagen type II and aggrecan were developed based on literature data [30], while primer sequences for collagen type I, SRY-box transcription factor 9, and glyceraldehydes-3-phosphate were developed using Primer-BLAST tool that is available online on the website of National Center for Biotechnology Information (NCBI) [31].
| Gene | Primer Sequence (5′–3′) |
Product Size (bp) |
|---|---|---|
| collagen type I (COL1A1) |
F: GGCCCAGAAGAACTGGTACA R: AATCCATCGGTCATGCTCTC |
81 |
| collagen type II (COL2A1) |
F: GGCAATAGCAGGTTCACGTACA R: CGATAACAGTCTTGCCCCACTTA |
79 |
| aggrecan (ACAN) |
F: AGCCTGCGCTCCAATGACT R: TAATGGAACACGATGCCTTTCA |
107 |
| SRY-box transcription factor 9 (SOX-9) |
F: GAGACTTCTGAACGAGAGCGA R: CGTTCTTCACCGACTTCCTCC |
125 |
| glyceraldehyde-3-Phosphate dehydrogenase (GAPDH) |
F: CACCACACTGAATCTCCCCT R: TGGTTGAGCACAGGGTACTT |
115 |