Table 1.
HPLC-MS/MS method parameters and performance.
| Analyte | Ion Pairs | DP * | CE * | CXP * | LOQ * | Linear Range | R2 |
|---|---|---|---|---|---|---|---|
| (m/z) | (V) | (V) | (V) | (ng/mL) | (ng/mL) | ||
| GSH | 433.2 → 304.1 | 46 | 20 | 20 | 0.01 | 0.01–500 | 0.9924 |
| GSSG | 613.2 → 355.2 | 116 | 29 | 24 | 5 | 5–500 | 0.9945 |
| Cys-Gly | 304.1 → 201.0 | 66 | 21 | 12 | 0.1 | 0.1–500 | 0.9992 |
| Glu-Cys | 376.1 → 247.1 | 71 | 21 | 12 | 0.05 | 0.05–500 | 0.9979 |
| Met | 150.2 → 103.8 | 41 | 15 | 8 | 0.1 | 0.1–500 | 0.9973 |
| Hcys | 261.1 → 55.8 | 51 | 41 | 8 | 0.05 | 0.05–500 | 0.9956 |
| Cys | 247.1 → 229.8 | 51 | 19 | 14 | 0.05 | 0.05–500 | 0.9926 |
* DP, declustering potential; CE, collision energy; CXP, collision cell exit potential; LOQ, limit of quantification (S/N >10).