Table 1.
The parameters of EPO-S100 interaction at 25 °C, estimated from the SPR spectroscopy data (Figure 1) using the heterogeneous ligand model (1). ka and kd refer to kinetic association and dissociation constants, respectively; Kd1 and Kd2 are equilibrium dissociation constants: Kd = kd/ka. The standard deviations are indicated.
| Parameter\Analyte | S100A2 | S100A6 | S100P |
|---|---|---|---|
| ka1, M−1s−1 | (2.2 ± 0.8) × 103 | (3.0 ± 0.6) × 103 | (2.0 ± 0.4) × 103 |
| kd1, s−1 | (1.8 ± 0.4) × 10−4 | (4.5 ± 0.5) × 10−4 | (1.10 ± 0.11) × 10−3 |
| Kd1, M | (8.1 ± 2.2) × 10−8 | (1.5 ± 0.3) × 10−7 | (5.4 ± 1.2) × 10−7 |
| ka2, M−1s−1 | (1.0 ± 0.5) × 104 | (2.5 ± 0.7) × 104 | (1.6 ± 0.5) × 104 |
| kd2, s−1 | (1.2 ± 0.5) × 10−2 | (1.6 ± 0.3) × 10−2 | (2.8 ± 0.4) × 10−2 |
| Kd2, M | (1.2 ± 0.7) × 10−6 | (6.5 ± 2.6) × 10−7 | (1.8 ± 0.5) × 10−6 |