Figure 7.

P3C decreased CREB, p38, ERK, and STAT3 and increased JNK and NF-kB phosphorylation in MDA-MB-231 cells after 3 h of exposure. Cellular extracts from cells exposed with 0.1 and 10 µM of P3C were analyzed with the antibody/bead-based Luminex xMAP technology. Control cells were exposed to solvent (0.2% v/v of DMSO) under the same conditions and analyzed concomitantly. The y-axis shows the median fluorescence intensity (MFI), and each error bar represents standard deviations of the mean of two independent measurements. Statistical significance was determined using Student’s t-test with values of p ≤ 0.05 deemed significant. Data acquirement and analysis were accomplished via xPONENT 3.1 software (Luminex).